禽多殺性巴氏桿菌可引起禽出血性敗血癥，又稱為禽巴氏桿菌病、禽霍亂，發(fā)病快，死亡率高。為對(duì)禽多殺性巴氏桿菌的致病機(jī)制研究及臨床防治提供支持，通過(guò)PCR鑒定、耐藥性分析、生物被膜形成能力試驗(yàn)以及半數(shù)致死量（LD50）測(cè)定，對(duì)4株臨床分離的雞源多殺性巴氏桿菌（Pm01、Pm03、1801及1803）進(jìn)行了相關(guān)生物學(xué)特性分析。PCR鑒定結(jié)果顯示，4株多殺性巴氏桿菌均為莢膜血清A型，均含有pfhA、exBDtonB、nanB、oma87、ompH、hgbB、hgbA、sodC、sodA 9種毒力基因，不含有toxA、nanH、ptfA 3種毒力基因；耐藥性分析結(jié)果顯示，4株多殺性巴氏桿菌對(duì)克林霉素、紅霉素、四環(huán)素、卡那霉素、阿莫西林、頭孢噻吩和氨芐西林等7種抗生素表現(xiàn)出全部或部分耐藥；生物被膜形成能力試驗(yàn)結(jié)果顯示，Pm01菌株能形成極強(qiáng)的生物被膜，而Pm03、1801以及1803菌株幾乎不能形成生物被膜；LD50測(cè)定結(jié)果顯示，4菌株LD50分別為1.33×101、2.74×102、4.22和4.22 CFU。結(jié)果表明，4株雞源多殺性巴氏桿菌含有多種毒力基因，均具有較強(qiáng)的致病性，其毒力與生物被膜形成能力不完全相關(guān)，且對(duì)多種抗生素耐藥。本研究為禽巴氏桿菌病防治提供了技術(shù)支撐與參考數(shù)據(jù)。
Analysis on the Biological Characteristics of Four Strains of Pasteurella multocida Derived from Chicken
Avian hemorrhagic septicemia，also known as avian pasteurellosis or avian cholera，is caused by Pasteurella multocida，with rapid occurrence and high mortality. In order to support future researches on the pathogenic mechanism of the bacteria as well as prevention and control of the disease in practice，relevant biological characteristics of four strains of Pasteurella multocida（Pm01，Pm03，1801 and 1803）were analyzed through PCR identification，drug resistance analysis，biofilm formation ability test and median lethal dose（LD50）determination. It was showed that，by PCR identification，all the four strains were capsular serotype A and contained 9 virulence genes including pfhA，exBDtonB，nanB，oma87，ompH，hgbB，hgbA，sodC and sodA，except toxA，nanH and ptfA；by drug resistance analysis，the four strains were fully or partially resistant to 7 antibiotics including clindamycin，erythromycin，tetracycline，kanamycin，amoxicillin，cefotaxime and ampicillin；by biofilm formation ability test，a very strong biofilm was formed by Pm01 strain，but failed by Pm03，1801 and 1803；and by the LD50 determination test，the LD50 values of the four strains were 1.33×101，2.74×102，4.22 and 4.22 CFU，respectively. In conclusion，all the four strains contained a variety of virulence genes that were with strong pathogenicity，for which the virulence was not completely related to the biofilm formation ability，and resistant to a variety of antibiotics. A technical support and reference data were provided for future prevention and control of avian pasteurellosis.
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