科研動態(tài)

牛結節(jié)性皮膚病（lumpy skin disease，LSD）是由牛結節(jié)性皮膚病病毒（lumpy skin disease virus，LSDV）引起的急性、亞急性傳染病。我國于2019 年8 月在新疆伊犁地區(qū)首次確診該病?？焖贉蚀_檢測是防控該病的關鍵。目前，應用常規(guī)PCR、實時熒光定量PCR、高分辨率熔解曲線分析、環(huán)介導等溫擴增等分子檢測方法，已實現(xiàn)了LSDV與其同屬不同種病毒的通用檢測和鑒別檢測以及LSDV野毒株與疫苗株的區(qū)分；應用病毒中和試驗、酶聯(lián)免疫吸附（ELISA）和蛋白印跡試驗（WB）等免疫學方法，可進行羊痘病毒屬的特異性抗原與抗體檢測，而對于LSDV特異性抗體檢測方法尚有待研究。LSD作為一種新傳入我國的外來動物疫病，有必要運用快速準確的分子或免疫學檢測方法加強監(jiān)測。本文概述了LSD診斷方法研究進展，為我國LSD診斷技術研發(fā)提供參考。

Research Progress on Diagnostic Methods of Lumpy Skin Disease

Lumpy skin disease（LSD），an acute and subacute infectious disease，is caused by lumpy skin disease virus（LSDV）. In August 2019，Chinese first case was confirmed in Yili Prefecture，Xinjiang. To prevent and control the disease is subject to rapid and accurate detection. Presently，the goal of generic detection of LSDV and its congeneric virus of different species as well as differential diagnosis of wild strains and vaccine strains have been achieved by a series of molecular detection methods，such as conventional PCR，real-time fluorescent quantitative PCR，high-resolution melting（HRM）curve analysis，loop mediated isothermal amplification（LAMP）；the specific antigen and antibody of capripoxvirus could be detected by the immunological methods，such as virus neutralization test（VNT），enzyme-linked immunosorbent assay（ELISA），Western Blot（WB），etc. However，the detection methods for specific antibodies against LSDV still need further studies. As a new exotic animal disease，LSD should be further monitored by rapid and accurate molecular or immunological methods. In conclusion，relevant research progress was summarized for the purpose of providing some references for the development of LSD diagnosis technology in China.

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