2019年4月，山東省新泰市某育肥豬場(chǎng)從某種豬場(chǎng)購進(jìn)了100頭5周齡仔豬。購進(jìn)1周后，有40多頭仔豬表現(xiàn)昏睡、嘔吐、拉稀等癥狀并出現(xiàn)急性死亡，共死亡28頭，病死率高達(dá)68%。對(duì)病豬進(jìn)行病理剖檢、組織病理學(xué)檢查、病原學(xué)檢測(cè)等實(shí)驗(yàn)室綜合診斷，最終確診為偽狂犬病病毒和副豬嗜血桿菌的混合感染。對(duì)分離的偽狂犬病病毒gE基因進(jìn)行測(cè)序和遺傳進(jìn)化分析，確定該分離株與近期流行株同源性較高，這為該病疫苗的選擇和高效使用提供了依據(jù)。基于診斷結(jié)果，制定了相應(yīng)的防控措施，使疫情得到了迅速控制。本文還分析討論了該病例的發(fā)生原因、發(fā)病特點(diǎn)及病變特征，對(duì)類似病例的診斷防控具有一定的指導(dǎo)意義。
Comprehensive Diagnosis of Mixed Infection with PorcinePseudorabies Virus and Haemophilus parasuis and Phylogenetic Analysis on the gEGene of Infected Strain
In April 2019，100 pigletsof 5-week old were purchased from a breeding farm by a fattening farm in XintaiCity of Shandong Province. Subsequently，some symptomssuch as lethargy，vomiting，diarrheaor even death appeared in more than 40 piglets after one week，a total of 28 piglets died，with a mortalityrate of 68%，which was finally confirmed as the mixedinfection with pseudorabies virus and Haemophilus parasuis through laboratorycomprehensive diagnosis including pathological autopsy，histopathologicalexamination and etiological test. Then the gE gene of isolated pseudorabiesvirus was sequenced and analyzed，and high homology ofthe isolate with current epidemic strain was found，whichprovided a basis for selection and efficient application of the vaccine. Basedon the above results，corresponding control measureswere formulated，and the outbreak was rapidlycontrolled. Furthermore，the reasons，clinical characters and pathological changes of the cases werediscussed and analyzed in the paper，which were ofsignificance for diagnosis and control of similar cases.
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