為了分析弓形蟲(chóng)GT1蟲(chóng)株GRA15（GRA15GT1）蛋白的反應(yīng)原性，通過(guò)PCR擴(kuò)增編碼GRA15GT1 52~635氨基酸肽段的基因片段，構(gòu)建pGEX-6P-1-GRA15GT1載體，轉(zhuǎn)化BL21菌誘導(dǎo)表達(dá)；通過(guò)SDS-PAGE和Western blot方法進(jìn)行表達(dá)驗(yàn)證及反應(yīng)原性分析。結(jié)果顯示：SDS-PAGE及以GST標(biāo)簽抗體為一抗進(jìn)行Western blot，均有目的條帶，比理論值稍大；以豬弓形蟲(chóng)陽(yáng)性血清為一抗的Western blot條帶與GST抗體孵育后的條帶大小一致。上述結(jié)果表明，GRA15GT1蛋白具有較好的反應(yīng)原性，這為下一步分段表達(dá)GRA15GT1蛋白，研究其在弓形蟲(chóng)血清學(xué)分型中的應(yīng)用奠定了基礎(chǔ)。
Expression and Immunoreactivity Exploration of GRA15Protein
of Toxoplasma gondii GT1 Strain
In order to analyze the immunoreactivity of GRA15protein of Toxoplasma gondii GT1 strain（GRA15GT1），the gene encoding a 584-residue peptide（from aa52 to aa635）was amplified by PCR，then the pGEX-6P-1-GRA15GT1 vector wasconstructed and transformed into BL21 strain of E. coli. After inducibleexpression，the purified GRA15 protein were obtainedand verified by SDS-PAGE and Western blot. Results showed that，the target strip was slightly larger thanits estimated size when the GST-tag mouse monoclonal antibody was used asprimary antibodies，as well as the swine anti-T. gondii positive serum. The resultsindicated GRA15GT1 protein had good immunoreactivity，which would lay a foundation for the next stepof GRA15GT1 protein expression and its application in serological typing ofToxoplasma gondii.
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